大阪歯科大学 教員情報

発表言語	英語
発表タイトル	Adipocytokine influences differentiation of osteoblasts
学会名	The 4th Sino-Japanese Conference on Stomatology 2008
発表形式	ポスター掲示
発表者・共同発表者	Kamada A, Ikeo T, Yoshikawa Y, Domae E, Goda S, Tamura I, Kawamoto A, Okazaki J, Komasa Y, Toda I, Suwa F
発表年月	2008/09/29
開催地	Xi’an, China
学会抄録	「Program & Abstracts of the 4th Sino-Japanese Conference on Stomatology 2008」　pp.92-93 「Program & Abstracts of the 4th Sino-Japanese Conference on Stomatology 2008.」　pp.92-93
概要	Objectives: Adipose tissue is not simply a store of excess energy, but also secretes a variety of proteins into circulating blood that influence systemic metabolism. Adipocytokines are a group of adipocyte-derived biologically active molecules, including adiponectin, leptin, tumor necrosis factor-alpha (TNF-alpha), resistin. A proteolytic cleavage product of adiponectin/Acrp30, gAcrp30, is found to circulate in plasma and to have a three-dimensional structure similar to that of TNF-alpha. In this study, we demonstrated the expression of adiponectin receptors in cultured pro-osteoblastic cells, and investigated the influences of gAcrp30 and TNF-alpha on osteoblast differentiation. Methodology: Gene Expression of adiponectin receptors were analyzed by RT-PCR method. Confluent monolayers of osteoblasts were precultured in alpha-MEM containing 0.1% serum for 24 h, and cultured in alpha-MEM containing 0.1% serum, ascorbic acid and beta-glycerophosphate supplemented with recombinant murine gAcrp30 or TNF-alpha for 24-48 h. The mRNA expression levels were determined by Quantitative Real Time PCR. Results: RT-PCR analysis demonstrated the expression of transcripts for both adiponectin receptors, AdipoR1 and AdipoR2, in murine pro-osteoblastic cells MC3T3-E1. Both gAcrp30 and TNF-alpha suppressed mRNA levels of type I collagen and RANKL compared with time-matched controls. Interestingly, gAcrp30 had no significant influence on the promotion of osteocalcin expression induced by the stimulation of ascorbic acid and beta-glycerophosphate, although TNF-alpha inhibited the increase of osteocalcin expression. Conclusion: These results indicated that adiponectin may not affect osteoblast differentiation but may suppress osteoclastogenesis in an endocrine manner, while TNF-alpha may inhibit the differentiation of pro-osteoblastic cells.