大阪歯科大学 教員情報

発表言語	英語
発表タイトル	Heparan sulfate proteoglycans modulate osteogenic differentiation through heparan sulfate chains
学会名	The 30th Annual Meeting of the American Society for Bone and Mineral Research
発表形式	ポスター掲示
発表者・共同発表者	Kamada A, Ikeo T, Yoshikawa Y, Domae E, Goda S, Tamura I, Kawamoto A, Okazaki J, Komasa Y, Takaishi Y, Miki T, Fujita T
発表年月	2008/09/15
開催地	Montreal, CANADA
学会抄録	「J Bone Miner Res」　23(suppl 1):s397
概要	Bone cells are regulated by interaction with growth factors and components of the extracellular matrix. Cell surface heparan sulfate proteoglycans (HSPGs), syndecan family and glypican family, are known to bind both other extracellular matrix molecules and certain growth factors, such as fibroblast growth factor (FGF), transforming growth factor (TGF) and bone morphogenetic protein (BMP), thus playing a unique role as a regulator of growth factor behavior. In this study, we investigated the role of HSPG in osteogenic differentiation of murine pro-osteoblastic cell line MC3T3-E1 cells using the HSdegrading endoglycosidase, heparitinase. Cells were pretreatment with heparitinase or chondroitinase ABC, and were stimulated by ascorbic acid (VC) and beta-glycerophosphate (BGP) to induce osteoblastic differentiation. Mineralization of the cell cultures was detected by von Kossa stain and by Alizarin Red. The mRNA expression level was measured using real-time quantitative RTPCR. Enzymatic depletion of the HS chains by heparitinase treatment enhanced the mineralization induced by VC and BGP. VC and BGP-dependent induction of osteocalcin increased significantly by heparitinase treatment compared with non-digested controls (p<0.01). Heparitinase treatment also enhanced the expression of bone-specific transcription factor Runx2 induced by BMP-2 addition, but not the BMP-2 expression. These results demonstrate that cell surface HSPGs suppress the mineralization in osteoblasts through HS side chain-mediated binding to BMP-2. Hence the control of cell surface HSPGs may be useful for modulate osteogenic differentiation of osteoblasts.