大阪歯科大学 教員情報

発表言語	英語
発表タイトル	Various roles of syndecan family in osteoblastic cells
学会名	The 30th Annual Meeting of the American Society for Bone and Mineral Research
発表形式	ポスター掲示
発表者・共同発表者	Ikeo T, Kamada A, Yoshikawa Y, Domae E, Goda S, Tamura I, Kawamoto A, Okazaki J, Komasa Y, Takaishi Y, Miki T, Fujita T
発表年月	2008/09/13
開催地	Montreal, CANADA
学会抄録	「J Bone Miner Res」　23(suppl 1):s162
概要	Syndecans are transmembrane heparan sulfate proteoglycans known to bind both other extracellular matrix molecules and certain growth factors, thus participating in a number of fundamental biological processes, including cell adhesion and migration, and the binding and activation of growth factors, enzymes, and inhibitors. The syndecan family in vertebrates comprises four members, syndecan-1, syndecan-2 (fibroglycan), syndecan-3 (N-syndecan), and syndecan-4 (ryudocan). Previously, we showed both protein and gene expression of syndecan-1, -2 and -4 in normal human osteoblasts. To understand the role of syndecans in osteoblasts, the effect of a bone- resorbing cytokine, IL-1beta, and differentiation-stimulated reagent, beta-glycerophosphate (BGP), on syndecan expression were examined using human osteoblast-like cell SaOS2. Using western blot analysis, it was found that IL-1b caused a marked appearance of a certain heparan sulfate proteoglycan corresponding to syndecan-4. Furthermore, the mRNA expression levels of syndecan family were measured using real-time quantitative RT-PCR technique, and demonstrated the gene expression of syndecan-1, -2 and -4 in the human osteoblast-like cells. When cells were incubated with IL-1beta for 24 h, there were significant decrease in mRNA levels of syndecan-1 and syndecan-2 compared with time match controls, though the expression of syndecan-4 was markedly increased. These results demonstrate that syndecan-1, -2 and -4 are synthesized in human osteoblastic cells, and syndecan-4 gene transcription is rapidly responsive to an inflammatory mediator. On the other hand, addition of BGP increased syndecan-1 and -2 expression but not syndecan-4.Hence, it is indicated that syndecan-1 and -2 may be involved with the differentiation and maturation of osteoblasts, while syndecan-4 may be important in the intracellular signal transduction.