| 論文種別 |
原著 |
| 記述言語 |
英文 |
| 表題 |
Influence of adipocytokines on differentiation of pro-osteoblastic cells |
| 掲載誌名 |
Journal of Oral Tissue Engineering
(ISSN:13489623) |
| 巻（号）：頁 |
6(1):17-23 |
| 著者・共著者名 |
Kamada A, Ikeo T, Yoshikawa Y, Domae E, Goda S, Tamura I, Kon-I H, Okazaki J, Kawamoto A, Komasa Y, Toda I, Suwa F |
| 発行年月 |
2008/09 |
| 概要 |
Adipocytokines are a group of adipocyte-derived biologically active molecules, including adiponectin, leptin, tumor necrosis factor-alfa (TNF-alfa), resistin. A proteolytic cleavage product of adiponectin/Acrp30, gAcrp30, is found to circulate in plasma and to have a three-dimensional structure similar to that of TNF-alfa. In this study, we demonstrated the expression of adiponectin receptors in cultured pro-osteoblastic cells, and investigated the influences of gAcrp30 and TNF-alfa on osteoblast differentiation.
RT-PCR analysis demonstrated the expression of transcripts for both adiponectin receptors, AdipoR1 and AdipoR2, in murine pro-osteoblastic cells MC3T3-E1. Both gAcrp30 and TNF-alfa suppressed mRNA levels of type I collagen and RANKL compared with time-matched controls. Interestingly, gAcrp30 had no significant influence on the promotion of osteocalcin expression induced by the stimulation of ascorbic acid and beta-glycerophosphate, although TNF-alfa inhibited the increase of osteocalcin expression.
These results indicated that adiponectin may not affect osteoblast differentiation but may suppress osteoclastogenesis in an endocrine manner, while TNF-alfa may inhibit the differentiation of pro-osteoblastic cells. |
| DOI |
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