| 論文種別 |
原著 |
| 記述言語 |
英文 |
| 表題 |
Emdogain stimulates matrix degradation by osteoblasts |
| 掲載誌名 |
Journal of Dental Research
(ISSN:00220345/15440591) |
| 巻（号）：頁 |
87(8):782-787 |
| 著者・共著者名 |
Goda S, Inoue H, Kaneshita Y, Nagano Y, Ikeo T, Iida J, Domae N |
| 発行年月 |
2008/08 |
| 概要 |
Emdogain has been used clinically for periodontal regeneration, although the underlying molecular mechanisms are not clear at present. In this study, we hypothesized that Emdogain stimulated degradation of type I collagen via osteoblasts. We showed that Emdogain enhanced cell-mediated degradation of type I collagen in an MMP-dependent manner. Although MG-63 cells spontaneously produced a zymogen form of MMP-1, treatment with Emdogain significantly induced the generation of the active form of this enzyme. We demonstrated that MMP-3 was produced from MG63 cells in response to Emdogain in a MEK1/2-dependent manner. Concomitantly, blocking of MEK1/2 activation by U0126 significantly inhibited the generation of the active form of MMP-1 without affecting the total production of this collagenase. These results suggest that Emdogain facilitates tissue regeneration through the activation of the collagenase, MMP-1, that degrades matrix proteins in bone tissue microenvironments. |
| DOI |
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