| 論文種別 |
原著 |
| 記述言語 |
英文 |
| 表題 |
Identification of Active Components in Connarus ruber Extract Exhibiting Anti-Glycation Effects |
| 掲載誌名 |
Medicines
(ISSN:23056320) |
| 巻（号）：頁 |
12(4):29 |
| 著者・共著者名 |
Taniguchi R, Nakatsuka R, Sasaki Y, Takenokuchi M, Maoka T, Iseki T, Kubo H, Nozaki T |
| 発行年月 |
2025/12 |
| 概要 |
Background: Glycation, a non-enzymatic reaction between sugars and biomolecules, leads to the formation of advanced glycation end-products (AGEs), which are implicated in the progression of chronic diseases. Connarus ruber (Poepp.) Planch (C. ruber), a traditional medicinal plant used for diabetes, has shown anti-glycation activity. This study aimed to identify the active components in C. ruber extract and elucidate their anti-glycation mechanisms. Methods: Using NMR and LC-MS analyses, we identified epicatechin and procyanidin A2 as major polyphenolic constituents. Collagen glycation assays were performed to evaluate the inhibitory effects of these compounds on fructose- and glyceraldehyde (GA)-induced glycation. Additionally, their cytoprotective effects were assessed using GA-induced cytotoxicity assays in dental pulp stem cells (DPSCs). Results: Both epicatechin and procyanidin A2 inhibited fructose- and GA-induced glycation in a dose-dependent manner, showing greater efficacy than aminoguanidine. Furthermore, these compounds significantly alleviated GA-induced cytotoxicity in DPSCs. Conclusions: These findings suggest that epicatechin and procyanidin A2 are candidate contributors to the anti-glycation and cytoprotective effects of C. ruber. The results support the potential of C. ruber extract as a source of therapeutic agents for glycation-related diseases and for enhancing stem cell viability. |
| DOI |
doi.org/10.3390/medicines12040029 |
